|Project Title||Next Generation Bovine Diagnostic Panel|
Next Generation Bovine Diagnostic Panel
· Multiplex diagnostic panel for bovine infectious disease
· Detects the presence of 45 of the most common pathogens
· A multiplicity of sample types can be used, including tissues, blood, milk and swabs
· More sensitive and specific than traditional culture-based diagnostics
· Can screen a sample for multiple pathogens simultaneously
· Easy data analysis
Diagnosis of infectious disease in cattle can be challenging, especially when the animal is infected with multiple pathogens. Traditionally infectious disease is diagnosed via the detection of organisms by bacterial culture, virus isolation, or antibody-based techniques. These methodologies have limitations, including the need for specialized staff, inefficiency in growth of cultures and need for proper sample handling.
The advantages of a nucleic acid-based technique, such as PCR, are numerous and include speed, sensitivity and specificity. However, PCR has its own challenges in that it is limited by pathogens that can be detected in a single reaction. To address that concern, UGA researchers have developed a Next Generation Sequencing (NGS)-based diagnostic panel that is able to detect the presence of 45 different pathogens using target-specific primers for PCR-mediated amplification. This multiplex panel has been validated using clinical samples that were subsequently tested with commonly used diagnostic techniques. These results confirm the validity of using NGS-based techniques in veterinary diagnostics.
• Rebecca, Wilkes, DVM, PH.D., DACVM
Assistant Professor, Department of Infectious Diseases
· Eman Anis, Ph.D.
Post-Doctoral Fellow, Department of Infectious Diseases
Technology Development and IP Status
· Primers have been developed and validated
· Test has proven to be efficacious in clinical samples
· Manuscript submitted for publication
· Technology available for know-how/trade secret license
|Tags||diagnostic, animal health|
|Posted Date||Jul 5, 2017 10:39 AM|