Details

Project TitleNext Generation Bovine Diagnostic Panel
Track Code2017-162
Short Description
Abstract

Next Generation Bovine Diagnostic Panel    

Applications

·         Multiplex diagnostic panel for bovine infectious disease

·         Detects the presence of 45 of the most common pathogens

·         A multiplicity of sample types can be used, including tissues, blood, milk and swabs

Problems Addressed

·         More sensitive and specific than traditional culture-based diagnostics

·         Can screen a sample for multiple pathogens simultaneously

·         Easy data analysis

·         Fast

·         Inexpensive

Pathogens Validated

Respiratory Pathogens

Enteric Pathogens

Repro Pathogens

Mastitis

BVDV (with typing), other pestiviruses, IBR (vs vaccine strain), Bovine Coronavirus, Mycoplasma species, Influenza D, PI3, BRSV, Adenovirus 3, Histophilus somni, Pasturella multocida, Mannheimia haemolytica, Trueperella pyogenes,  Bibersteinia trehalosi

E. coli toxins (F41, F5, sta, stx 1,2, eae, cnf 1/2, alpha hly) Salmonella, Rotavirus (A,B,C), Johne’s, Clostridium perfringens toxin typing (alpha, beta, beta2, epsilon, iota, cpe), Giardia, Cryptosporidium, Coronavirus

Neospora, Leptospirosis species, Toxoplasma, Chlamydia species, Campylobacter fetus fetus and venerealis, BHV-4, IBR, BVDV, Brucella abortus,

T. foetus, Bluetongue/EHD, Anaplasma marginale, Listeria monocytogenes, Ureaplasma

Staphylococcus aureus, E. coli toxins, Streptococcus agalactiae, Mycoplasma bovis, Prototheca, Streptococcus uberis, Streptococcus dysgalactiae,

Coag negative Staphylococcus, Pseudomonas, Klebsiella, Zygomycetes,  Aspergillus, Nocardia

Technology Summary

Diagnosis of infectious disease in cattle can be challenging, especially when the animal is infected with multiple pathogens. Traditionally infectious disease is diagnosed via the detection of organisms by bacterial culture, virus isolation, or antibody-based techniques. These methodologies have limitations, including the need for specialized staff, inefficiency in growth of cultures and need for proper sample handling.

The advantages of a nucleic acid-based technique, such as PCR, are numerous and include speed, sensitivity and specificity. However, PCR has its own challenges in that it is limited by pathogens that can be detected in a single reaction. To address that concern, UGA researchers have developed a Next Generation Sequencing (NGS)-based diagnostic panel that is able to detect the presence of 45 different pathogens using target-specific primers for PCR-mediated amplification. This multiplex panel has been validated using clinical samples that were subsequently tested with commonly used diagnostic techniques.  These results confirm the validity of using NGS-based techniques in veterinary diagnostics.

Inventors

•         Rebecca, Wilkes, DVM, PH.D., DACVM

Assistant Professor, Department of Infectious Diseases

·         Eman Anis, Ph.D.

Post-Doctoral Fellow, Department of Infectious Diseases

Technology Development and IP Status

·         Primers have been developed and validated

·         Test has proven to be efficacious in clinical samples

·         Manuscript submitted for publication

·         Technology available for know-how/trade secret license

 
Tagsdiagnostic, animal health
 
Posted DateJul 5, 2017 10:39 AM